Polarity of human parainfluenza virus type 3 infection in polarized human lung epithelial A549 cells: role of microfilament and microtubule.

Epithelial A549 Cells: Role of Microfilament 3 Infection in Polarized Human Lung Polarity of Human Parainfluenza Virus Type

Role of nucleolin in human parainfluenza virus type 3 infection of human lung epithelial cells.

Human parainfluenza virus type 3 (HPIV-3) is an airborne pathogen that infects human lung epithelial cells from the apical (luminal) plasma membrane domain. In the present study, we have identified cell surface-expressed nucleolin as a cellular cofactor required for the efficient cellular entry of HPIV-3 into human lung epithelial A549 cells. Nucleolin was enriched on the apical cell surface do...

Polarized and Non-Poarized Human Oviduct Epithelial Cell Ultrastructure in Vitro

Purpose: This study designed to examine polarized culture of epithelial cells from human ovidutc and their ultrastracture under polarizing condition. Materials and Methods: The human oviduct was obtained from patients having undergone total hysterectomy and epithelial cells were isolated using collagenase type I. The epithelial cells were either cultured on ECM (Extracellular matrix) Gel coate...

Evaluation of silica nanoparticles cytotoxicity (20-40 nm) on cancerous epithelial cell (A549) and fibroblasts cells of human normal lung fibroblast (MRC5)

Introduction: Silica nanoparticles have received more attraction in medical and industrial applications due to their unique properties such as small size, the possibility of surface functionalization, ease of production, and low cost. So, it is necessary to study the respiratory toxicity of occupational exposure due to the production and increasing use of silica nanoparticles, especially in the...

Filamentous particle formation by human parainfluenza virus type 2.

Some paramyxoviruses form long filamentous virus particles: however, the determinants of filament formation and the role of such particles in virus transmission and pathogenicity are not clearly defined. By using conventional immunofluorescence microscopy, we found that human parainfluenza virus type 2 (HPIV2) forms filamentous particles ranging from 5 to 15 microm in length in virus-infected, ...